Download e-book for iPad: Paradigms of Neural Injury by J. Regino Perez-Polo

By J. Regino Perez-Polo

ISBN-10: 0080536484

ISBN-13: 9780080536484

ISBN-10: 0121853004

ISBN-13: 9780121853006

This quantity contains paradigms, version structures, and methods for the learn of dysfunctions within the fearful method. the benefits and downsides of the methods awarded are severely mentioned. Key good points* Neural harm* Developmental mobile loss of life* disorder approaches and getting older

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It is barely soluble in buffer solutions and requires a 2hour preincubation before initiation of experiment. FK506, or immunophilin (Fujisawa Pharmaceuticals, Japan), binds to FKBP-12, and the FK506FKBP12 complex binds to calcineurin, inactivating the phosphatase. NOS is activated by calcineurin-mediated dephosphorylation. Arginine Depletion Arginine is the precursor to NO formation by NOS. Cell cultures can readily be depleted of arginine by two methods. 1. Culture cells for 24 hours in arginine-free MEM (GIBCO, Grand Island, NY, special order) in the presence of 2 mM glutamine to inhibit argininosuccinate synthase.

Wasson, D. , and Carrera, C. , Exp. Cell Res. 164, 273 (1986). , Free Rad. Biol. Med. 14, 389 (1993). , Drian, M. , Kamenka, J. , Brain Res. 617, 138 (1993). , Physiol. Rev. 59, 527 (1979). , NeuroReport 2, 121 (1991). Chiueh, C. , and Murphy, D. , Synapse 11, 346 (1992). Choi, D. , and Rothman, S. , Annu. Rev. Neurosci. 13, 171 (1990). Cleeter, M. J. , Cooper, J. , and Schapira, A. H. , J. Neurochem. 58, 786 (1992). , and Heikkila, R. , J. Biol. Chem. 249, 2447 (1974). , Biochem. J. 2, 1420 (1963).

Dev. Brain. Res. 20, 169 (1985). Wolff, S. , and Dean, R. , Biochem. J. 234, 399 (1986). , Ellis, H. , and Horvitz, H. , Cell 75, 641 (1993). , and Piantadosi, C. , J. Clin. Invest. 90, 1193 (1992). , Dawson, V. , Dawson, T. , and Snyder, S. , Science 263,687 (1994). , Neurosci. Lett. 170, 136 (1994). [2] Nitric Oxide Toxicity in Central Nervous System Cultures Valina L. Dawson* and Ted M. Dawson Introduction Primary neuronal cell cultures are ideal as a model system for investigating isolated cellular mechanisms in that they retain most of the physiological and biochemical characteristics of in situ neurons (1) and are free from the influences of the microvasculature.

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Paradigms of Neural Injury by J. Regino Perez-Polo

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